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KMID : 0895420100200010070
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Journal of Korean Society of Occupational and Enviromental Hygiene
2010 Volume.20 No. 1 p.70 ~ p.78
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A study on biomarker for biomonitoring of 1,3-butadiene inhalation exposure
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Lee Jin-Heon
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Abstract
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The purpose of this study is to investigate the appropriate metabolite as biomarker for the biomonitoring of 1,3-butadiene(BD) inhalation exposure. We measured the hemoglobin adducts which were extracted from the blood of the ICR mice inhalation exposure with 100ppm and 500ppm 1,3-butadiene for 2 weeks(5 hr/day × 5 days/week). Hemoglobin adducts were the (N-2-hydroxy-3 -butenyl) valine (HB Val) and (N-2,3,4-trihydroxy-butyl)valine (THB Val). Body weights of the exposure groups were significantly lower from 11 exposure post-day in 100ppm BD inhalation mice and from 7 exposure post-day in 500ppm BD inhalation mice than in control. The levels of HB Val are 0.8∼1.7pmol/mg globin for 100ppm BD inhalation exposure, and 2.1∼4.4 pmol/mg globin for 500ppm BD inhalation exposure. The levels of THB Val are 15.0∼22.0 pmol/mg globin in 100ppm BD inhalation exposure, and 34.8∼45.7 pmol/mg globin for 500ppm BD inhalation exposure. So the levels of THB Val and HB Val are proportional relationship with BD exposure level. THB Val is 12.9∼18.8 times higher level that HB Val in 100ppm BD exposure group and 10.4∼16.6 times higher level than HB Val in 500ppm BD exposure group. We concluded that THB Val is an appropriate metabolite as biomarker for the biomonitoring for BD inhalation exposure.
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KEYWORD
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1,3-butadiene, biomarker, (N-2-hydroxy-3-butenyl)valine(HB Val), (N-2,3,4-trihydroxy-butyl)valine(THB Val)
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